Immunohistochemistry of resorption and inflammation factors in the periodontal ligament of human deciduous teeth.

The understanding of the biological mechanisms involved in root resorption in deciduous teeth is important to the future development of preventive measures and treatments of this condition. The aim of the present study was to compare the expression and immunostaining of iNOS, MMP-9, OPG and RANKL in the periodontal ligament (PDL) of deciduous teeth with physiologic root resorption (GI), inflammatory pathological root resorption (GII) and permanent teeth (GIII), the negative control. Teeth in GI (n = 10), GII (n = 10) and (GIII) (n = 10) were submitted to immunohistochemical analysis to determine the expression of iNOS, MMP-9, OPG, and RANKL. The immunostaining was analysed by optical density. Statistical analysis included one-way ANOVA, followed by Student-Newman-Keuls post hoc test (p < 0.05). The results showed that iNOS, MMP-9 and RANKL expression in the PDL was higher in GII compared to GI and GIII (p < 0.05). Moreover, RANKL expression was higher in GI compared to GIII (p < 0.001), while OPG immunolabelling was lower in GII compared to GI and GIII (p < 0.001). The PDL of deciduous teeth bearing inflammatory processed exhibited upregulation of resorption-associated factors as well as enzymes related to tissue degradation which, in turn explains the exacerbation and greater susceptibility of those teeth to root resorption process.

Immunohistochemistry of resorption and inflammation factors in the periodontal ligament of human deciduous teeth

Abstract: The understanding of the biological mechanisms involved in root resorption in deciduous teeth is important to the future development of preventive measures and treatments of this condition. The aim of the present study was to compare the expression and immunostaining of iNOS, MMP-9, OPG and RANKL in the periodontal ligament (PDL) of deciduous teeth with physiologic root resorption (GI), inflammatory pathological root resorption (GII) and permanent teeth (GIII), the negative control. Teeth in GI (n = 10), GII (n = 10) and (GIII) (n = 10) were submitted to immunohistochemical analysis to determine the expression of iNOS, MMP-9, OPG, and RANKL. The immunostaining was analysed by optical density. Statistical analysis included one-way ANOVA, followed by Student-Newman-Keuls post hoc test (p < 0.05). The results showed that iNOS, MMP-9 and RANKL expression in the PDL was higher in GII compared to GI and GIII (p < 0.05). Moreover, RANKL expression was higher in GI compared to GIII (p < 0.001), while OPG immunolabelling was lower in GII compared to GI and GIII (p < 0.001). The PDL of deciduous teeth bearing inflammatory processed exhibited upregulation of resorption-associated factors as well as enzymes related to tissue degradation which, in turn explains the exacerbation and greater susceptibility of those teeth to root resorption process.

PLGA+HA/ßTCP Scaffold Incorporating Simvastatin: A Promising Biomaterial for Bone Tissue Engineering.

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite/ß-tricalcium phosphate (PLGA+HA/ßTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% ßTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m/m) of this medication. Scaffolds were synthesized in a cylindric shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in phosphate-buffered saline at 37°C under constant stirring for 7, 14, 21, and 28 days. Nondegraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro-, meso-, and micropores in the polymer structure with HA/ßTCP particles homogeneously dispersed. Chemical and thermal analyses presented similar results for both PLGA+HA/ßTCP and PLGA+HA/ßTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/ßTCP and PLGA+HA/ßTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/ßTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

Retrospective study of 124 cases of salivary gland tumors and literature review.

BACKGROUND: Salivary gland tumors are a rare and morphologically diverse group of lesions and their frequency is still unknown in several parts of the world. The knowledge of its population characteristics contributes to a better understanding of its etiopathogenesis. Objectives: This study investigated the frequency of salivary gland tumors in a region of southern Brazil and compared these data in a literature review. MATERIAL AND METHODS: A retrospective study was conducted of salivary gland tumors diagnosed at two pathology centers from 1995 to 2016. Patient age and gender, tumor site and frequency, histopathological diagnosis, and symptomatology were evaluated. Chi-squared tests were used to assess the associations between variables. To compare our data, we also conducted a literature review of publications in the PubMed and LILACS databases of retrospective studies of salivary gland tumors. RESULTS: A total of 124 salivary gland tumor cases was identified, 81 (65.3%) of which were classified as benign and 43 (34.6%) as malignant. Most tumors occurred in the parotid gland (57.2%). Pleomorphic adenoma was the most common tumor (59.6%), followed by adenocarcinoma not otherwise specified (8.8%). The tumors occurred more often in women (54.8%) than in men (45.2%). Malignant tumors were associated with pain in 31.4% of cases (p<0.05). The literature review included 35 articles from different countries. Women were most affected, with a mean age of 41.7 years. The most common benign tumor was pleomorphic adenoma (48.2%) and the most common malignant tumor was mucoepidermoid carcinoma (8.7%). CONCLUSIONS: The results of the present study showed that salivary gland tumors are rare. The parotid gland is the most common location and pleomorphic adenoma are the most frequent lesions. The malignant tumors presented as several histological types and the incidence was variable globally. Key words:Salivary gland neoplasms, salivary gland diseases, oral surgery, epidemiology.

Inhibition of cancer stem cells promoted by Pimozide.

Over the past years, studies have described that users of antipsychotics are less likely to develop cancer than the population in general due to cytotoxic properties of this class of drugs on cancer cells. For this reason, Pimozide has been widely studied as a potential anticancer treatment, and satisfactory results in melanoma, central nervous system tumours, osteosarcoma, neuroblastoma, myeloproliferative neoplasms, breast, lung, prostate, ovarian, colorectal, pancreatic, and hepatocellular carcinoma have been showed. Moreover, advantages as clinical use approved by the Food and Drug Administration (FDA), high clinical safety, low side effects, and reasonable price have stimulated the treatment with Pimozide instead of other agents. The action mechanism remains unclear, but three vias associated to cancer stem cell (CSC) hypothesis show that Pimozide: (a) blocks CSC features, as epithelial-to-mesenchymal transition (EMT), through inhibition of Wnt-ß/catenin signalling; (b) acts as an inhibitor of signal transducer and activator of transcription (STAT-3 and 5), pathway which is activated and up-regulated in CSCs; (c) inhibits ubiquitine specific protease (USP1) and WD repeat-containing protein 48 (WDR48), that are proteins responsible to inhibit the differentiation and to maintain the cell in an undifferentiated state. Based on this perspective, the aim of this manuscript is to review the antineoplastic role of Pimozide during tumorigenesis and its potential to revert the process of undifferentiation and proliferation of CSC through different vias.

Natal and neonatal teeth: two clinical cases report / Dentes natais e neonatais: relato de dois casos clínicos

ABSTRACT The aim of this study was to report two cases of newborns with natal and neonatal teeth. Case 1 - a female child presented a natal tooth in the region of lower central incisors since birth. Mother's child reported a family history of this feature. Since the tooth did not present either mobility, prevent breastfeeding, cause discomfort to the child or hurt mother's breast, it was chosen for its maintenance and monitoring. Case 2 - a male child presented, during the first two weeks of life, the eruption of two neonatal teeth in the region of lower central incisors. During the interview, his mother reported no familiar history of this condition. Because the teeth has tapered edges, causing the Riga-Fede's disease, impairing breastfeeding, causing discomfort to the child and injuring the mother's breast, it was decided for its extraction. It may be concluded that the presence of natal and neonatal teeth represent a rare condition that requires further studies to confirm its etiology. Therapeutic approaches must be preceded by a careful clinical evaluation since, as it was seen in this report, both cases were submitted to different treatments showing good evolution.

RESUMO O objetivo deste estudo foi relatar dois casos clínicos de recém-nascidos que apresentaram dentes natais e neonatais. Caso 1 - criança do gênero feminino, apresentou ao nascimento, um dente natal na região de incisivos centrais inferiores. Durante a anamnese, a mãe relatou histórico familiar desta alteração. Devido o elemento dentário não apresentar mobilidade, não impedir a amamentação, não causar desconforto à criança e não ferir a mama da mãe, optou-se pela sua manutenção e acompanhamento. Caso 2 - criança do gênero masculino, apresentou na primeira quinzena de vida, o irrompimento de dois dentes neonatais na região de incisivos centrais inferiores. Durante a anamnese, a mãe relatou não haver histórico familiar desta alteração. Devido a esses elementos dentários apresentarem bordas afiladas, causando a doença de Riga-Fede, comprometerem a amamentação, causarem desconforto à criança e ferirem a mama da mãe, optou-se pela sua exodontia. Conclui-se que a presença de dentes natais e neonatais é uma condição rara e mais estudos são necessários para se confirmar sua etiologia. As abordagens terapêuticas devem seguir uma criteriosa avaliação clínica, pois, como visto neste relato, os dois casos foram submetidos a diferentes tratamentos e ambos apresentaram boa evolução.

Teaching and Learning Regarding the Dentist-Pediatric Patient Relationship: A Qualitative Study

Objective: To investigate the perceptions of dental students on the learning process regarding the dentist-pediatric patient relationship. Material and Methods: A qualitative study with an exploratory approach was conducted involving semistructured interviews with sixteen dental undergraduate students randomly selected from the thirty-two students in the last semester of the dentistry course. The transcribed interviews were submitted to content analysis. Results: The sample was composed of nine female (56.3%) and seven male (43.7%) students (median age: 23 years). The factors perceived as most influential with regard to learning the dentistpediatric patient relationship were observations of professors, other dentists or colleagues and the regular practice of dental procedures. While half of the participants considered lectures on the subject satisfactory, the other half reported a need for more instruction on how to deal with specific clinical situations. Most of the participants would like to have learned more about the dentist-pediatric patient relationship. Conclusion: The majority of students recognized that the teaching and learning process regarding the dentist-pediatric patient relationship is not easy. They reported having little counseling on the how to develop this relationship. The students suggested that the dentist-patient relationship should be addressed more during the course through the inclusion of lectures or classes on this issue as well as the demonstration of specific clinical situations.

Crown discoloration promoted by materials used in regenerative endodontic procedures and effect of dental bleaching: spectrophotometric analysis.

OBJECTIVES: To assess tooth crown's color after intracanal treatment with triple antibiotic paste (TAP) or calcium hydroxide (CH); cervical sealing with glass ionomer cement (GIC) or mineral trioxide aggregate (MTA); and bleaching with carbamide peroxide. MATERIAL AND METHODS: After pulp removal and color spectrophotometer measurement, 50 bovine incisors were divided into 4 experimental groups and one control (untreated). Experiments were performed in phases (Ph). Ph1: TAP (ciprofloxacin, metronidazole, minocycline), TAPM (ciprofloxacin, metronidazole, amoxicillin), DAP (ciprofloxacin, metronidazole), or CH treatment groups. After 1 and 3 days (d); 1, 2, 3 weeks (w); and 1, 2, 3 and 4 months (m), color was measured and medications were removed. Ph2: GIC or MTA cervical sealing, each using half of the specimens from each group. Color was assessed after 1d, 3d; 1w, 2w, 3w; 1m and 2m. Ph3: Two bleaching sessions, each followed by color measurement. Data were analyzed with ANOVA and post-hoc Holm-Sidak method. RESULTS: Ph1: Specimens of TAP group presented higher color alteration (ΔE) mean than those of TAPM group. No significant difference was found among TAP or TAPM and CH, DAP or Control groups. Ph2: cervical sealing materials showed no influence on color alteration. Ph3: Different ΔE means (from different groups), prior to bleaching, became equivalent after one bleaching session. CONCLUSIONS: TAP induces higher color alteration than TAPM; color alteration increases over time; cervical sealing material has no influence on color alteration; and, dental bleaching was able to recover, at least partially, the tooth crown's color.

Crown discoloration promoted by materials used in regenerative endodontic procedures and effect of dental bleaching: spectrophotometric analysis

Abstract Regenerative endodontic procedure (REP) has been proposed as a new approach to treat immature permanent teeth. However, materials used in REP for root canal disinfection or cervical sealing may induce tooth discoloration. Objectives To assess tooth crown’s color after intracanal treatment with triple antibiotic paste (TAP) or calcium hydroxide (CH); cervical sealing with glass ionomer cement (GIC) or mineral trioxide aggregate (MTA); and bleaching with carbamide peroxide. Material and Methods After pulp removal and color spectrophotometer measurement, 50 bovine incisors were divided into 4 experimental groups and one control (untreated). Experiments were performed in phases (Ph). Ph1: TAP (ciprofloxacin, metronidazole, minocycline), TAPM (ciprofloxacin, metronidazole, amoxicillin), DAP (ciprofloxacin, metronidazole), or CH treatment groups. After 1 and 3 days (d); 1, 2, 3 weeks (w); and 1, 2, 3 and 4 months (m), color was measured and medications were removed. Ph2: GIC or MTA cervical sealing, each using half of the specimens from each group. Color was assessed after 1d, 3d; 1w, 2w, 3w; 1m and 2m. Ph3: Two bleaching sessions, each followed by color measurement. Data were analyzed with ANOVA and post-hoc Holm-Sidak method. Results Ph1: Specimens of TAP group presented higher color alteration (ΔE) mean than those of TAPM group. No significant difference was found among TAP or TAPM and CH, DAP or Control groups. Ph2: cervical sealing materials showed no influence on color alteration. Ph3: Different ΔE means (from different groups), prior to bleaching, became equivalent after one bleaching session. Conclusions TAP induces higher color alteration than TAPM; color alteration increases over time; cervical sealing material has no influence on color alteration; and, dental bleaching was able to recover, at least partially, the tooth crown’s color.

The Role of the Complex USP1/WDR48 in Differentiation and Proliferation Processes in Cancer Stem Cells.

BACKGROUND: Recently, some studies identified the Basic-Helix-Loop-Helix (bHLH) transcription factor as a significant regulator for the evolution of neoplasms. The binding between bHLH proteins and DNA is restricted by heterodimerization with Inhibitors of DNA binding (ID). IDs prevent cellular differentiation, promote growth and sustain tumor development. The wide presence of stem cells in cancers suggests that genes ID are essential to cancer stem cells (CSC) progress. The enzyme Ubiquitin-specific protease 1 (USP1) is reported to deubiquitinate and stabilize IDs. Considering the action of the proteins ID, USP1 contributes to prevent differentiation mediated by bHLH and, consequently, keep CSC original characteristics. USP1 has its activity potentiated when bound to protein WD repeat-containing protein (WDR48). OBJECTIVE: To identify the influence of the complex USP1/WDR48 during the CSC tumorigenesis process, and whether this complex is a possible therapeutic target. METHODS: A literature search regarding the role of the complex USP1/WDR48 in inhibiting differentiation and increasing proliferation of CSC was performed, and possible selective molecule inhibitors of these deubiquitinase proteins were investigated. RESULTS: There is evidence that USP1/WDR48 complex promotes stem cell conservation and regulation of DNA damage repair. For this reason, inhibitors as Pimozide, GW7647, C527, SJB2-043, ML323 have been studied to inhibit USPs in cases of treatment intervention. CONCLUSION: It is consolidated in the literature the role of USP1/WDR48 during tumorigenesis. However, these studies are not enough to completely clarify the process; but certainly, the researchers are converging towards a promising direction to provide a new treatment option for cancer.

Avaliação radiográfica e em microscopica eletrônica de varredura de remanescentes de obturação após a re-instrumentação endodôntica / Radiographic and scanning electron microscopic assessment of root canal filling remnants after endodontic re-instrumentation

Objetivo: Falhas no tratamento endodôntico podem ocorrer por várias razões. O retratamento endodôntico é uma alternativa interessante para tratar esse problema clínico. No entanto, não é possível remover completamente o material obturador do canal radicular por qualquer das técnicas atuais de retratamento. O objetivo deste estudo foi avaliar a presença de resíduos de material obturador após a re-instrumentação endodôntica. Material e Métodos: Sessenta dentes anteriores humanos foram preparados pela técnica escalonada com Flexofiles, limas K e brocas GatesGlidden (GG). Entre o uso de cada instrumento ou broca, os canais radiculares foram irrigados com hipoclorito de sódio (NaOCl). A lama dentinária foi removida por irrigação com ácido etilenodiaminotetraacético e NaOCl. Após secagem com pontas de papel, os canais radiculares foram divididos aleatoriamente em 5 grupos (n = 12), de acordo com o material obturador: cones Resilon/cimento Real Seal ou cones de guta-percha e cimento Endofill, Sealapex, AH Plus ou MTA Fillapex. Após uma semana, o material obturador foi removido usando Eucaliptol e K-files. Os canais radiculares foram re-instrumentados com limas K e brocas GG de tamanhos maiores do que as anteriormente usadas. A presença de remanescente de material obturador no canal radicular foi analisada por radiografia e microscopia eletrônica de varredura (MEV). A análise estatística foi realizada pelo teste de Regressão Logística Binária (P < 0,05). Resultados: As análises radiográfica e por MEV mostraram que o material do grupo MTA Fillapex foi melhor removido do que o dos grupos Endofill, Sealapex, AH Plus e Real Seal. Conclusão: Após a re-instrumentação, o grupo MTA Fillapex apresentou menos remanescentes nos canais radiculares que os grupos Endofill, Sealapex, AH Plus e Real Seal. O material residual foi encontrado mais frequentemente no terço apical. (AU)

Objective: Failures in endodontic treatment may occur by several reasons. Endodontic retreatment is an interesting alternative to manage this clinical problem. However, it is not possible to completely remove the root canal filling by any current retreatment technique. The aim of this study was to evaluate the presence of residual root canal filling materials after endodontic reinstrumentation. Material and Methods: Sixty extracted anterior human teeth were prepared by step-back technique with Flexofiles, K-files and Gates-Glidden (GG) burs. Between the use of each file or bur, root canals were irrigated with sodium hypochlorite (NaOCl). Smear layer was removed by irrigation with ethylenediaminetetraacetic acid and NaOCl. After drying with paper points the root canals were randomly divided into 5 groups (n = 12), according to filling material: Resilon cones/Real Seal sealer or gutta-percha cones and Endofill, Sealapex, AH Plus or MTA Fillapex sealers. After one week, root canal fillings were removed using Eucaliptol and K-files. Root canals were reinstrumented with K-files and GG burs sized larger than the first ones. The removal of root canal filling material was analyzed by radiography and scanning electron microscopy (SEM). Statistical analysis was performed using Binary Logistic Regression test (P < 0.05). Results: Radiographic and SEM analysis showed that material from the MTA Fillapex group was better removed than that from Endofill, Sealapex, AH Plus and Real Seal groups. Conclusion: After re-instrumentation, MTA Fillapex group showed less remnants into the root canals than Endofill, Sealapex, AH Plus and Real Seal groups. Residual material was most often found in the apical third(AU)

Analysis of Bone Repair and Inflammatory Process Caused by Simvastatin Combined With PLGA+HA+ßTCP Scaffold.

PURPOSE: This study evaluated the tissue and inflammatory responses to the use of simvastatin and poly(lactic-co-glycolic acid) + hydroxyapatite + ß-tricalcium phosphate (PLGA+HA+ßTCP) scaffold for bone repair. MATERIALS AND METHODS: Two defects of 5 mm in diameter were made in the calvaria of rats, which were shared into the following 6 groups: naive, sham, vehicle, PLGA+HA+ßTCP scaffold, simvastatin (4 mg/mL), and simvastatin with the scaffold. Tissue samples were collected at 1, 7, 15, 30, and 60 days after surgery. Inflammation was evaluated by interleukin-1 beta and tumor necrosis factor alpha quantification and by a hemogram, whereas bone repair was evaluated using densitometry and scanning electron microscopy. Data were statistically analyzed using ANOVA followed by post hoc tests (P < 0.05). RESULTS: There was an increased cytokine expression in the scaffold and simvastatin groups (P < 0.001 and P < 0.05, respectively) 1 day after surgery but no alterations on the hemogram were observed. It was found on bone tissue samples that 60 days after surgery all groups presented similar densitometry values and morphology characteristics, despite the occurrence of bone formation delay in the simvastatin group (P < 0.01). CONCLUSION: The use of simvastatin and PLGA+HA+ßTCP scaffold, associated or not, did not lead to improvement in bone repair.

Immunohistochemical expression of matrix metalloproteinases in ameloblastomas and pericoronal follicles.

BACKGROUND: Ameloblastoma (AM) is a benign odontogenic neoplasm characterized by local invasiveness and recurrence. We compared the immunohistochemical expression of matrix metalloproteinases in different clinical types of AM as well as in normal odontogenic tissue. METHODS: Thirteen cases of solid AMs, five cases of unicystic AM and eight pericoronal follicles (PF) were selected and subjected to immunohistochemical investigation for matrix metalloproteinase-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 expressions. RESULTS: The expressions of MMP-1 and MMP-2 were very high in the cytoplasm of cells throughout the entire epithelium and in fibroblasts from the adjacent connective tissue. MMP-9 expression was observed in the same location although with weaker staining. The Kruskal-Wallis test showed statistically significant differences in the epithelial expressions of MMP-1 and MMP-2; there was lower expression among solid AMs when compared with unicystic AM and PF. Compared to both types of AM, higher stromal expression of MMP-9 was found in PF. CONCLUSION: MMP-1, MMP-2 and MMP-9 seem to be associated with AM tumour behaviour as well as physiological tissue remodelling within PF.

Knowledge of stem cells among Dentistry undergraduates

Introduction:Stem cells (SCs) enabled great advances in different health fields, including Dentistry. Objective: To investigate the knowledge of last year Dentistry undergraduate students regarding SCs and its applications. Material and methods: A questionnaire with open-ended questions was applied to the students, followed by qualitative-quantitative analysis involving the clustering of the answers. Results: The majority of students (70%) defined SCs adequately, only half of the interviewees (48.8%) were unable to classify SCs and just 26.2% classified it correctly. On the question about SCs from dental tissues, 77.5% reported it was possible and 70.9% knew which tissues could be used. The main source of knowledge was the media (36.4%), followed by the University (26.6%); 88.5% stated that the topic was only addressed in the first year of the course. Consequently, only 7.5% reported being capable of answering questions about it. Conclusion:The students are not receiving enough information about the topic.

A versatilidade do uso da tuberosidade maxilar para pequenas reconstruções teciduais na Implantodontia / The versatility of the use of maxillary tuberosity for small tissue reconstructions in Implant Dentistry

As alterações alveolares após a exodontia de um elemento dental desafiam a Implantodontia na busca por técnicas e materiais que devolvam ao rebordo alveolar os tecidos perdidos com previsibilidade e menor morbidade ao paciente. Este relato de casos clínicos mostra a instalação de implantes imediato ou tardio, associados ou não ao procedimento de carga imediata utilizando o enxerto misto da tuberosidade maxilar como alternativa viável para essas reconstruções, tendo como objetivo principal a redução do número de procedimentos cirúrgicos e a previsibilidade no período pós operatório. O osso da tuberosidade maxilar é composto por uma fina camada de osso cortical externo e medular interno que apresentam alta capacidade de revascularização e integração à área receptora. A vantagem em se manter o periósteo entre o enxerto corticomedular do túber e o tecido conjuntivo é conter a reabsorção óssea periosteal na região cervical e melhorar a qualidade do tecido mole. A limitação do uso da tuberosidade maxilar está relacionada a sua dimensão, que pode permitir a reconstrução de um ou dois dentes por área doadora ou, em alguns casos, pode não haver área doadora disponível. As características únicas da tuberosidade maxilar de fornecer osso corticomedular e tecido conjuntivo subepitelial permitem a sua associação no enxerto misto de túber e mostra grande versatilidade para a reconstrução dos tecidos duros e moles, permitindo técnicas refinadas capazes de solucionar casos complexos em um único procedimento cirúrgico...

Alveolar alterations after extraction constitute a challenge and lead to the search for techniques and materials to reconstruct the lost tissues with previsibility and less morbidity to the patients. These case report shows the placement of immediate implants, delayed implants, with or without the immediate loading procedure and maxillary sinus lift with immediate implant placement using the maxillary tuberosity mixed graft as a viable alternative to these reconstructions aiming to reduce the number of surgical procedures and improve predictability in the post-operative period. The tuberosity jaw bone consists of a thin outer layer of cortical bone and inner medullary bone that have a high capacity of revascularization and integrating into the receiving area. The advantage of keeping the periosteum between tuberosity corticomedular graft and the connective tissue is to contain the periosteal bone resorption in the cervical area and improve the quality of the soft tissue. Limitation to the use of maxillary tuberosity is related to their dimensions that may allow the reconstruction of one or two teeth per donor area or, in some cases, it may not be available. The unique characteristics of the maxillary tuberosity to provide corticomedular bone and subepitelial connective tissue allow their association in the maxillary tuberosity mixed graft and show great versatility for the reconstruction of hard and soft tissue, allowing refined techniques to solve complex cases in a single surgical procedure...

Dentigerous cyst of inflammatory origin.

There is an association between persistent, prolonged inflammation of a primary tooth and the development of an inflammatory dentigerous cyst involving the succedaneous tooth. The purpose of this case report is to describe the management of an inflammatory dentigerous cyst of the permanent maxillary left central incisor in a nine-year-old boy caused by a long-term inflammation/infection of its predecessor. The treatment consisted of conservative decompression, which allowed for rapid healing and the eruption of the permanent tooth. The patient was followed up with periodic clinical and radiographic evaluations for several years.

Microbial reduction by two chemical-mechanical protocols in primary teeth with pulp necrosis and periradicular lesion - an in vivo study.

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x10(5) cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.

Knowledge of Human Genome Project among Dentistry undergraduates

Introduction: The Human Genome Project (HGP) has allowed for advances in diagnosis and prevention of diseases. Objective: The aim of this study was to investigate the knowledge of undergraduates from the Federal University of Santa Catarina, regarding HGP and its applicability. Material and methods: Thirty-one students were interviewed by using a questionnaire with open questions, followed by qualitative and quantitative analysis through answers' grouping. Results: Only 4.5% did not know HGP, while most of them (83%) demonstrated knowledge gained from the media. Only 21% cited the undergraduate program as a source of information, 56% recognized advances in disease prevention and 81% reported applicability in Dentistry. 97% students would give blood samples for research, 28% reported concern about misuse of this information, while 31% did not report any disadvantage. Conclusion: It may be concluded that the students have some knowledge on HGP. However, it is not widely discussed during course, because the media was the main source of information.

Microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion.

PURPOSE: The purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion. METHODS: Microbial samples were collected from 24 canals of children treated at a pediatric dentistry clinic. Microbiological identification was performed using checker-board DNA-DNA hybridization for 40 different bacteria. Data were analyzed per canal based on the mean count and frequency of each bacterial species. RESULTS: Detectable levels of bacterial species were observed for 35 probes (88%). The most frequent bacteria were Fusobacterium nucleatum sp. nucleatum, Fusobacterium periodonticum, Prevotella melaninogenica, Prevotella nigrescens, and Prevotella intermedia. Facultative species were identified in 20 root canals (83%), anaerobic species were identified in 24 root canals (100%), and aerobic species in 18 root canals (75%). Black-pigmented bacilli were found in 23 samples (96%). The number of different bacterial species detected per canal ranged from five to 33. CONCLUSION: Endodontic infection in primary teeth with pulp necrosis and periradicular lesion is multimicrobial, including aerobic, facultative, and anaerobic micro-organisms.

Microbial Reduction by Two Chemical-Mechanical Protocols in Primary Teeth with Pulp Necrosis and Periradicular Lesion - An In Vivo Study

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x105 cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.

O objetivo deste estudo foi determinar a eficácia das manobras químico-mecânicas de dois protocolos endodônticos, na redução do conteúdo séptico de canais radiculares de dentes decíduos com polpa necrosada e lesão perirradicular. Vinte e quatro canais radiculares decíduos com necrose pulpar e lesão perirradicular foram divididos em dois grupos de tratamento (n=12): multisessões e sessão única. Amostras foram coletadas usando pontas de papel estéreis, antes e após a limpeza endodôntica, seguido de identificação microbiológica por hibridização DNA-DNA checkerboard. A análise estatística foi realizada usando teste de proporções para escore=0, comparando os achados antes e após tratamento para cada grupo (teste de Wilcoxon) e as diferenças dos escores entre os protocolos (teste de Mann-Whitney) (p<0,05). Os dados foram expressos em prevalência (presença ou ausência) e contagem média (x105 células) de cada espécie. As diferenças nas proporções de escore=0 antes do tratamento não foram significativas (p=0,415), mostrando equivalência entre os grupos. Um aumento significativo de escore=0 foi detectado após o tratamento para ambos os grupos (p<0,0001). O protocolo de sessão única mostrou uma redução significativamente maior dos escores médios após o tratamento endodôntico (p=0,024). Ambos os protocolos são capazes de reduzir significativamente o conteúdo séptico de canais radiculares de dentes decíduos com lesão perirradicular. Entretanto, o protocolo de sessão única mostrou uma maior eficácia na redução da infecção endodôntica.